And its use yeast cell surface display of protein

摘要

(57) in the form accessible for binding to the polymer, [summary] The present invention provides a genetic method for anchoring the polypeptide in the yeast cell wall. Combination with the method and the fluorescence activated cell sorting provides a means for selecting specific affinity was reduced or increased to another molecule, altered protein or with a condition of bond. There is also provided a method for genetically fused to the C terminus of the yeast Aga2p cell wall protein, the N-terminus of the polypeptide of interest. be attached to the Aga2p agglutinin, a scFv antibody fragment, effectively mimic the cell surface display of antibodies by B cells in the immune system against affinity maturation in vivo. T cell receptor mutants displayed efficiently on the yeast cell surface is isolated by this method, which may provide a means of T cell receptor binding affinity and alter the specificity of the library screening. The selection method may, to identify (proteins secreted protein that appears at a higher level, more efficiently, proteins and more stable) proteins with phenotypic features enhanced.