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TRANSFORMABLE RHODOBACTER STRAINS, METHOD FOR PRODUCING TRANSFORMABLE RHODOBACTER STRAINS

机译:可转化的根瘤菌菌株,生产可转化的根瘤菌菌株的方法

摘要

The invention provides an organism for expressing foreign DNA, the organism engineered to accept standard DNA carriers. The genome of the organism codes for intracytoplasmic membranes and features an interruption in at least one of the genes coding for restriction enzymes. Further provided is a system for producing biological materials comprising: selecting a vehicle to carry DNA which codes for the biological materials; determining sites on the vehicle's DNA sequence susceptible to restriction enzyme cleavage; choosing an organism to accept the vehicle based on that organism not acting upon at least one of said vehicle's sites; engineering said vehicle to contain said DNA; thereby creating a synthetic vector; and causing the synthetic vector to enter the organism so as cause expression of said DNA.
机译:本发明提供了用于表达外源DNA的生物,该生物被工程化为接受标准DNA载体。生物体的基因组编码胞浆内膜,其特征在于至少一种编码限制酶的基因被打断。还提供了一种用于生产生物材料的系统,该系统包括:选择载体以携带编码该生物材料的DNA;确定在车辆DNA序列上易受限制酶切割的位点;基于一种生物体,该生物体不作用于所述车辆的至少一个地点,从而选择一种生物体来接受该车辆;工程化所述载体以包含所述DNA;从而产生合成载体;使合成载体进入生物体,以引起所述DNA的表达。

著录项

  • 公开/公告号US2014080176A1

    专利类型

  • 公开/公告日2014-03-20

    原文格式PDF

  • 申请/专利权人 PHILIP D. LAIBLE;DEBORAH K. HANSON;

    申请/专利号US201213619406

  • 发明设计人 PHILIP D. LAIBLE;DEBORAH K. HANSON;

    申请日2012-09-14

  • 分类号C12N1/21;C12P21/00;C12P1/04;C12N15/74;C12M1/42;

  • 国家 US

  • 入库时间 2022-08-21 16:08:01

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