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METHOD AND DEVICE FOR MONITORING REAL-TIME POLYMERASE CHAIN REACTION (PCR) UTILIZING ELECTRO-ACTIVE HYDROLYSIS PROBE (E-TAG PROBE)
METHOD AND DEVICE FOR MONITORING REAL-TIME POLYMERASE CHAIN REACTION (PCR) UTILIZING ELECTRO-ACTIVE HYDROLYSIS PROBE (E-TAG PROBE)
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机译:利用电活性水解探针(E-TAG探针)监测实时聚合酶链反应(PCR)的方法和装置
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摘要
A method for real-time electrochemical monitoring of PCR amplicons using a hydrolysis probe that is labeled with electro-active indicators and a microchip for implementing the method. The method provided is simpler and has higher specificity compared with the prior art. The electrochemical signal measured during the PCR process can be used to determine the initial amount of the target DNA. This technique can be applied in detection and quantification of nucleic acids, especially for point-of-use applications such as on-site nucleic acid-based bio-analysis.
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