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Culture medium and method for inducing differentiation into adipocytes

机译:培养基和诱导分化为脂肪细胞的方法

摘要

Disclosed are a culture medium, an additive, and a method for efficiently inducing the differentiation of mammalian somatic stem cells into cells having the characteristics of adipocytes under conditions of serum-free or low-serum culture medium. The culture medium for inducing the differentiation of mammalian somatic stem cells into adipocytes comprises a basal medium for culturing mammalian cells, an agent for inducing the differentiation of mammalian somatic stem cells into adipocytes, biotin, a ligand for endothelial cell differentiation gene (Edg) family receptors, vitamin C, and HEPES, which culture medium is serum-free or contains a low concentration of serum.
机译:公开了一种在无血清或低血清培养基的条件下有效诱导哺乳动物体干细胞向具有脂肪细胞特征的细胞分化的培养基,添加剂和方法。用于诱导哺乳动物体干细胞向脂肪细胞分化的培养基包括用于培养哺乳动物细胞的基础培养基,用于诱导哺乳动物体干细胞向脂肪细胞分化的试剂,生物素,内皮细胞分化基因(Edg)家族的配体。受体,维生素C和HEPES,这些培养基不含血清或含有低浓度的血清。

著录项

  • 公开/公告号US8785199B2

    专利类型

  • 公开/公告日2014-07-22

    原文格式PDF

  • 申请/专利权人 YOSHIYUKI HOTTA;

    申请/专利号US201013202720

  • 发明设计人 YOSHIYUKI HOTTA;

    申请日2010-02-18

  • 分类号C12N5/00;C12N5/077;

  • 国家 US

  • 入库时间 2022-08-21 16:03:21

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