Hyaluronic Acid-Gelatin Crosslinked Thermoreversible Pluronic Hydrogels

摘要

A HA-gelatin-pluronic hydrogel and a method for making a HA-gelatin-pluronic hydrogel are provided. The hydrogel includes hyaluronic acid, gelatin-Type A and a thermoreversible, hydrophilic non-ionic surfactant gel. The thermoreversible, hydrophilic non-ionic surfactant gel can be a poly(ethylene oxide)99-poly(propylene oxide)67-poly(ethylene oxide)99 (“Pluronic F127”). The weight ratio of HA to gelatin-Type A is between 1:2 and 2:1 and preferably about 1:1. The weight ratio of HA and gelatin-Type A to thermoreversible, hydrophilic non-ionic surfactant gel is between 1:3,000 and 1:150, preferably between 1:3,000 and 1:600 and most preferably about 1:2,400. The hydrogel is formed by combining hyaluronic acid and gelatin-Type A to form a solution, cooling the solution, mixing the solution with Pluronic F127 to form a mixture and storing the mixture at a temperature of from 25° C. to 45° C. preferably about 37° C., to form a gel