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Method for measuring bonding activity of antibody which mimics antibody-dependent cell medicated cytotoxic activity
Method for measuring bonding activity of antibody which mimics antibody-dependent cell medicated cytotoxic activity
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机译:模仿抗体依赖性细胞介导的细胞毒活性的抗体结合活性的测定方法
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摘要
The present invention provides a simple method which is capable of evaluating the binding activities of an antibody to both an antigen and an Fe receptor. Disclosed is a method of measuring the binding activities of an antibody to both an antigen or antigen epitope and an Fc receptor or a fragment thereof, comprising a step of mixing the antibody with the antigen or antigen epitope labeled with one member of a set of donor and acceptor capable of fluorescent resonance energy transfer and the Fc receptor or fragment thereof labeled with the other member of the set of donor and acceptor; a step of 10 irradiating the resultant mixture with light having a wavelength capable of exciting the donor; and a step of measuring the fluorescence level of the mixture. Also provided are a method of estimating the ADCC activity of an antibody, a method of controlling the quality of an antibody, a method of manufacturing an antibody, a method of screening for antibodies, and kits for use in these methods.
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