The process of obtaining of the biological 5.7 - dihydroxy-7,7 - 6.4 - dimetoxiflavona and product development with the potential citostu00c1tico against cu00c2ncer of human ovu00c1rio

摘要

The process of obtaining of the biological 5.7 - dihydroxy-7,7 - 6.4 - dimetoxiflavona and product development with the potential citostu00c1tico against cu00c2ncer of ovu00c1rio human.The present invention falls within the sector, chemical pharmaceutical, referring to the development of a methodology for obtaining a chemical class of flavonoids agliconas active in the formulated product against cells of human ovarian cancer.The presenteinvenu00e7u00e3o shows a process of obtaining the substance 5,7 - dihydroxy-7,7 6.4 39 - dimetoxiflavona (active principle) from tissues of the orchid Miltonia flavescens cultivated in greenhouse, the use of procedures of extraction, partition chromatography, purification and recrista Lilzau00e7u00e3o.This invention also features the use of a formulation against sarcoma of human ovary (line NCI / ADR - RES) working as a cytostatic agent at a concentration of GI ~ 50 ~ = 2,1 109 G / ml of active principle. The formulation presented lower toxicity against cells of human normal control (line HaCaT, Im ~ 50 ~ = 18.5 109 G / ml).Outrossiim, the object of the present invention formulation is considerably less toxic to normal cells compared to the formulation containing the standard chemotherapy of experiment (doxorubicin, Im ~ 50 ~ = 0.028 198 G / ml) and presents an active principle with the chemical structure less complex Xa compared to paclitaxel.The reference in the treatment of chemotherapy of ovarian cancer is available in the market.