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Processes and kits for deteu00c7u00c3o of resistance to rifampin and / or rifabutin species of Mycobacterium.
Processes and kits for deteu00c7u00c3o of resistance to rifampin and / or rifabutin species of Mycobacterium.
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机译:用于检测对分枝杆菌的利福平和/或利福布丁物种的抗性的方法和试剂盒。
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摘要
Method for the detection of the antibiotic resistance spectrum of Mycobacterium species present in a sample, possibly coupled to the identification of the Micobacterium species involved, comprising the steps of: (i) if need be releasing, isolating or concentrating the polynucleic acids present in the sample; (ii) if need be amplifying the relevant part of the antibiotic resistance genes present in said sample with at least one suitable primer pair; (iii) hybridizing the polynucleic acids of step (i) or (ii) with at least one of the rpoB gene probes, as specified in table 2, under the appropriate hybridization and wash conditions; (iv) detecting the hybrids formed in step (iii); (v) inferring the Mycobacterium antibiotic resistance spectrum, and possibly the Mycobacterium species involved from the differential hybridization signal(s) obtained in step (iv).
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