Method for detecting the presence of a at least one target, at least two targets or a apluralidade targets, method for detection of a first facilitator assembly using a cascade, the use of a pair of nuclu00c9icos acids isolated MNA ligase enzyme and its use, the product of the enzyme Miss ligase, U. So at least two olingonucleotu00cddeos as substrates.

摘要

The present invention relates to utilize the method for polycomponent nucleic acid complex (MNA complex body) cascade.The MNA complex body can have the activity of cutting or ligase enzyme.Further, the invention provides the cascade that can comprise one or more DNA ribozymes.The present invention also provides evaluation, detection and the quantitative methods of using these cascades to be used for target.