A PROCESS FOR THE PREPARATION OF PROTEIN MEDIATED CALCIUM HYDROXYAPATITE (HAP) COATING ON METAL SUBSTRATES

摘要

This invention provides a process for the preparation of protein mediated calcium hydroxyapatite (HAp) coating on metal substrates particularly on stainless steel (316 L) by biomimetic route, capable of rapid and effective osteointegration with the host tissue following controlled interfacial reactions. For the purpose, calcium deficient, carbonated hydroxyapatite (HAp) coating was developed on metal substrates particularly on stainless steel (316L) alloy through biomimetic route after a surface treatment step using aqueous solution (8-10 wt %) of BSA at room temperature. The coating was characterised with respect to phase composition, crystallinity, morphology and thickness. The protein mediated calcium phosphate ceramic coating is porous (pore dia-100-200µm) having uniform pore distribution and coverage. There is multifold enhancement in thickness and crystallinity (data provided) of the as-prepared coating. The process in the present invention is designed to mimic the structural and characteristic properties of the biological apatite to expedite osteointegration kinetics and to further improve biocompatibility of the metallic implant.