Method for preparing the formulation aid of enzymes environmental bioremediation of hydrocarbon contaminated heavy crude oil and a method for bioremediation of contaminated environment heavy crude oil

摘要

method for enzyme preparation supporting bioremediacju0119 environments contaminated p31 heavy fraction of crude oil.by culture of fungus aspergillus niger strain nitkowatego ibt - 150, is stored on a medium containing activation strain brzeczku0119 piwowarsku0105, agar agar and heavy fraction oil p31, then washing the cultured spores saline with added surfactantvaccination received inoculum liquid substrate and the breeding breeding production temperature 29 - 31 degrees during the 96 - 120 hours on a shaker, and then drained obtained biomass, washing it with distilled water, acetone dehydration, drying and mechanical rozdrobieniu.method of soil contaminated p31 bioremediation heavy fraction oil is the preparation of inoculum the single strain or strains of bacteria consortium properties degradation of hydrocarbons, by culture of shake to wysterylizowanym liquid containing substrate available source of carbon, nitrogen, phosphorus and growth substances.then the namnou017conego inoculum in soil and the culture swept under aerobic conditions, during which, between 12 - 16 day bioremediation to oczyszczanego environment is suspended in sterile water preparation of enzymes.a bioremediation aquatic environment affected heavy fraction oil p31 is preparing a single strain or strains of bacteria inoculum consortium properties degradation hydrocarbons by culture on liquid medium wysterylizowanym shake containing available source of carbon, nitrogen, phosphorus and growth substances.then the namnou017conego inoculum in soil and the culture swept under aerobic conditions, during which, between 7 - 10 day to oczyszczanego bioremediation, environment is suspended in sterile water preparation of enzymes.