Method of production of haploid plants, double haploids and / or dihaploids, by gynogenesis

摘要

The invention relates to a process for producing haploid H, doubled haploid HD and / or dihaploids DH, with the HD and DH homozygous or essentially homozygous, this being a type of procedure regarding the technique gynogenesis pollen induced irradiated. This method comprises a step of irradiating the reproductive material from the male parent with a dose of between 160 and 190 gamma ray and / or a step of selecting the haploid H and / or DH using marker (s) molecular (s ). The invention also relates to a method of production of haploid plants, double haploids and / or homozygous dihaploid comprising a step of determining (or the) dose suitable irradiation (s) to increase yields of these plants depending multiple factors given as the plant species, genotype from the male parent and the female parent, the climatic and physiological conditions, the time of harvest of the fruit, the level of growth of embryos collected with a view to its cultivation, the level development of embryos placed in culture. Moreover, the invention relates to (the) marker (s) molecular (s) (s) used in the selection step and the haploid embryos, dihaploid embryos obtained by the process of the invention, the offspring and plant seeds obtained by the process of the invention.