Method for determining glicosintasa activity and use thereof in high productivity screening libraries enzymes with activity glicosintasa

摘要

Method for determining glicosintasa activity and use thereof in screening high-productivity libraries enzymes glicosintasa activity # The present invention relates to a method of detecting glicosintasa activity in a sample comprising:. Mixing the sample with a glycosyl fluoride and optionally with a glycoside acceptor, incubating, add a silyl ether of formula (I), incubating and measuring fluorescence. This method can be used in selecting glicosintasas.