Method for identifying molecular techniques of genetic variants that do not encode antigen D (D) and encode altered antigen C (C + W)

摘要

A method for differentiating variants RHD-CE * DIIIa (4-7) -D blood group or type RHD-CE * DIIIa (4-7) -D, expressing the antigen C + W and lack of antigen D , RHD * DIIIa, RHD * DIVA-2 and other variants gruposanguíneo of a subject, the method comprising: determining at least four markers in a sample obtained from the subject, wherein the markers comprise: (i) the presence or absence of an allele of RHCE * C; (Ii) the presence or absence of an allele of exon 3 hybrid RHD / RHCE (RHD / EC Hex03); (Iii) the absence of, or a variant polymorphism single nucleotide (SNP) within, any one delexón 4 RHD RHD exon or RHD exon 6; and (iv) the absence of, or SNP variant within, RHD exon 7, wherein: the absence of said allele RHCE * C; the presence of said allele of exon 3 hybrid RHD / RHCE; laausencia RHD exon 4, exon 5 or exon 6 RHD RHD; and the absence of exon 7 RHD encombinación indicate that the sample contains said variant RHD-CE * DIIIa blood group (4-7) -D odicha variant blood group type RHD-CE * DIIIa (4-7) -D .