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Device for measuring light, which comes from particles or from biological cells of microscopic size

机译:用于测量来自微粒或微小尺寸生物细胞的光的设备

摘要

Device for measuring the light emitted from particles or cells (12) of microscopic size, with a passage cell (8), through which the particles or luminous cells (12) are conducted, in which the cell step (8) presents a window transparent to light, and with a photo detector (2), which detects the light emitted from the particles or luminous cells (12), and with an optical element, which conducts the light that comes out from the window (3) towards the photo detector (2), in which the optical element is configured as a cylinder (4, 9) with a cylindrical reflection surface, in which the passage cuvette (8) is arranged outside the cylinder ( 4, 9), such that the cylinder (4, 9) is arranged between the passage cuvette (8) and the photo detector (2), in which the passage cuvette (8) is arranged with its longitudinal axis perpendicular to the longitudinal axis of the cylinder (4, 9), and in which the device is configured to measure the light fluo Resident that comes from particles or cells (12) of microscopic size, in which between the passage cuvette (8) and the photo detector (2) is a fluorescent light filter (6) that lets in fluorescent light, the device it is free of lenses, and the cylinder (4, 9) has a large numerical aperture, where the diameter of the cylindrical reflection surface is at least 50 times greater than the minimum distance between the light emitting particle and the inlet opening of the cylinder light (4, 9), where the cylindrical reflection surface is formed by a cylinder liner (4, 9).
机译:用于测量从微观尺寸的颗粒或细胞(12)发出的光的装置,该细胞带有通过通道(8)的颗粒或发光细胞(12),通道步骤(8)在其中具有透明的窗口光检测器(2)检测从颗粒或发光细胞(12)发出的光,光学元件将从窗口(3)出来的光传导到光检测器(2),其中光学元件被配置为具有圆柱形反射面的圆柱体(4、9),其中通过比色杯(8)布置在圆柱体(4、9)的外部,使得圆柱体(4) ,9)布置在通道比色皿(8)和光检测器(2)之间,其中通道比色皿(8)的纵轴垂直于圆柱(4、9)的纵轴,并且该设备配置为测量来自粒子或细胞的荧光通量(12 )的尺寸,其中在比色杯(8)和光检测器(2)之间是荧光滤光片(6),该荧光滤光片(6)允许荧光,没有透镜的装置,以及圆柱体(4、9) )具有较大的数值孔径,其中圆柱反射表面的直径至少是发光粒子与圆柱光(4、9)的入口之间的最小距离的50倍以上。由气缸套(4、9)形成。

著录项

  • 公开/公告号ES2494016T3

    专利类型

  • 公开/公告日2014-09-12

    原文格式PDF

  • 申请/专利权人 PARTEC GMBH;

    申请/专利号ES20040715875T

  • 发明设计人 GÖHDE WOLFGANG;

    申请日2004-03-01

  • 分类号G01N15/14;G01N21/64;

  • 国家 ES

  • 入库时间 2022-08-21 15:55:01

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