System for specific selection of cells and state of development of embryonic stem cells differentiating adult non-human, non-human embryonic stem cells and stem cells

摘要

Method for obtaining differentiated cells or stem cells differentiating into a specific cell type, comprising: (a) introducing into stem cells, except human embryonic stem cells, at least one or two vectors containing DNA sequences containing the information for at least one reporter gene encoding a fluorescent protein does not damage the cells and at least one resistance gene; wherein both DNA sequences are under control of the same cell-specific promoter and / or development it is operably linked to said genes; (B) culturing the cells under conditions allowing differentiation in the desired cell type; (C) detecting the expression of said reporter gene; (D) adding a selective agent for selecting those cells expressing the reporter gene and the resistance gene; (E) obtaining the differentiated or differentiating cells developing from said stem cells into a specific cell type under the control of cell-specific promoter and / or development.