Microfluidic apparatus and method for DNA extraction, amplification and analysis

摘要

An integrated gel based microfluidic sample processing device An integrated gel based microfluidic sample processing device, suitable for forensic investigations at the scene of a crime,comprising a substrate having a plurality of micro-channels to form at least a DNA extraction chamber in fluidic cooperation with an amplification chamber which in turn is in fluidic cooperation with a separation and detection channel; said micro-channels containing a DNA extraction material and gel based reaction reagents necessary for processing said sample; the device further comprising electrical contacts for coupling to an external power source and capable of inducing electro-kinetic manipulation of said gel based reagents and DNA extracted from said sample throughout the device.