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METHOD AND KIT FOR QUANTIFYING MATERIAL OF BOVINE AND BUBALINE ORIGIN IN PRODUCTS OF ANIMAL ORIGIN

机译:动物源产品中牛源和胆碱源材料的定量方法和试剂盒

摘要

The present invention describes a method and kit for quantifying material of bovine and bubaline origin in milk or meat products and other products of animal origin, using a group of specific primers and probe. In order to quantify DNA, the TaqMan real-time PCR system is used as the standard, but the method is also suitable for the SYBR Green PCR system. The present invention further describes a new method for standardising the total quantity of DNA present in a given sample, and plotting calibration curves according to the type of material under analysis.
机译:本发明描述了使用一组特异性引物和探针定量牛奶或肉类产品和其他动物源性产品中牛和胆碱类物质的方法和试剂盒。为了定量DNA,以TaqMan实时PCR系统为标准,但该方法也适用于SYBR Green PCR系统。本发明进一步描述了一种新方法,该方法用于标准化给定样品中存在的DNA总量,并根据被分析材料的类型绘制校准曲线。

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