Gene inactivation allowing immediate growth on xylose medium by engineered zymomonas

摘要

Zymomonas cells that are genetically engineered to have a disrupted aldose reductase gene such that aldose reductase activity for conversion of xylose to xylitol in the presence of NADPH is reduced by greater than 90%, and that are engineered to express a xylose utilization metabolic pathway, were found to have the ability to gro on medium containing xylose as the only sugar without adaptation in media containing xylose.