COMPREHENSIVE PROMOTER AND USE THEREOF IN CANCER GENE THERAPY

摘要

The invention relates to biotechnology, in particular to genetic engineering, and constitutes a comprehensive promoter (alternatives) which is active in more than three types of cancer cells of varying etiology, said promoter being more active than a native promoter of the BIRC5 gene and comprising a combination of recognition sites of transcription factor proteins, said combination being changed or extended in comparison to the initial promoters. The invention also relates to an expression vector comprising said promoter, to a method for selective killing of cancer cells but not of normal cells, resulting in toxin synthesis within the cancer cells with the use of the promoter, and also to a method for treating cancer in mammals by introducing an animal vector. The invention makes it possible to direct enzyme and cytokine synthesis with the use of the promoter according to the invention in such a manner that a prodrug which is deliverable externally is converted into a toxin, and to activate the cells of the immune system.