Polyacrylamide precast gels for electrophoresis, process for producing the same and electrophoresis method by using the gels

摘要

A precast acrylamide electrophoresis gel is disclosed, which includes a gel buffer solution containing either Tris and glycine or Tris, glycine and any one or more than two of ampholytes, and a monobasic acid, titrated to a specified pH range. With the gels constituted as stated earlier, proteins can be successfully subjected to electrophoresis using the commonly available Laemmli's buffer system, while the DNA can be also satisfactorily run on the gel using the electrophoresis electrode solution containing Tris, chelating agent, and any one acid of acetic acid, phosphoric acid and boric acid. In either of protein and DNA analyses, the gels even after stored under refrigeration more than six months are fully comparable in resolution and clearness of electrophoretic pattern to the gels just after being prepared. Where the DAN is subjected to electrophoresis using either the Laemmli's buffer or the Ornstein and Davis buffer, commonly used in electrophoresis of proteins, the gels even after storage under refrigeration over four months may exhibit the electrophoretic patterns comparable in resolution and clearness to the gels soon after being produced.