RECOMBINANT PROTEIN OFHAEMONCHUSCONTORTUS AND USE THEREOF FOR THE PRODUCTION OF A VACCINE AGAINST HAEMONCHOSIS

摘要

A recombinant protein (rHc23) of H. contortus was produced after establishing that the native protein Hc23, with a molecular weight of 23kDa, originating from a soluble extract from the adult haematophagous nematode H. contortus demonstrated an immunoprotective ability in young sheep of 5 months of age against the most significant trichostrongylidosis (haemonchosis) from an economic and pathological point of view. Protein Hc23 was characterised, obtaining genome and amino acid sequences of same, which, using molecular biology techniques, recombinant DNA, can be used to produce a stable protein, rHc23. The use thereof in immunogenic compositions that include, as an adjuvant, the combination of inactivated Propionibacterium acnes + lipopolysaccharide of E. coli has demonstrated a high degree of protection in animals (protection 85%).