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Improved transfection of eukaryotic cells with linear polynucleotides by electroporation
Improved transfection of eukaryotic cells with linear polynucleotides by electroporation
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机译:通过电穿孔改善线性多核苷酸对真核细胞的转染
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摘要
The present invention provides an improved method for gene delivery in eukaryontic cells by electroporation, preferably in human hematopoietic cells, particular dendritic cells. The method of the invention is superior to lipofection and passive pulsing of mRNA and to electroporation of plasmid cDNA for gene delivery, including tumor antigen loading of dendritic cells.
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