FLUORESCENCE POLARIZATION ASSAY FOR DETECTING HIGH MOLECULAR WEIGHT MOLECULES IN BIOLOGICAL FLUIDS

摘要

A point of care (POC) device measures the concentration of large molecular weight molecules or particles in a small volume of complex fluid using a fluorescence polarization assay where binding of a tagged probe molecule to a target molecule is detected as an increase in anisotropy of emitted fluorescent light. Autofluorescence is selectively reduced from the signal by time-gating the emission detection to select for emissions from long-lived fluorophores. This minimizes an otherwise intolerable discordance across population due to variable autofluorescence.