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GENE ANALYSIS METHOD, AND DEVICE AND KIT FOR ANALYSIS OF GENE

机译:基因分析方法以及用于基因分析的装置和工具包

摘要

A DNA sequencer is a device for analyzing a nucleotide sequence, and is not intended for the detection of a polymorphism that occurs in a trace amount. Furthermore, the crosstalk at the wavelength of a fluorescent substance to be used for the labeling of DNA interferes with the acquisition of low signals on a single coordinate. Therefore, it has been difficult to detect the mutation of a gene having a small abundance ratio, such as the mutation in a somatic cell. A gene analysis device is equipped with: multiple flow paths which respectively correspond to nucleotide species in a nucleic acid sample in which the nucleotides are labeled separately; a wave form data generation section for detecting a label signal from each of the nucleotides in the nucleic acid sample that is placed in each of the multiple flow paths, thereby generating wave form data on the intensity of the detected signal; a peak detection section for detecting a peak value of the wave form data for each of the nucleotides; and a data integration section for carrying out an integration processing of multiple sets of wave form data.
机译:DNA测序仪是一种用于分析核苷酸序列的设备,并不旨在检测痕量的多态性。此外,用于DNA标记的荧光物质的波长处的串扰会干扰在单个坐标上获取低信号。因此,难以检测出丰度比小的基因的突变,例如体细胞的突变。基因分析装置具备:分别与分别标记了核苷酸的核酸样品中的核苷酸种类对应的多个流路。波形数据产生部分,用于从置于多个流路中的每一个中的核酸样品中的每个核苷酸检测标记信号,从而产生关于检测到的信号强度的波形数据;峰值检测部分,用于检测每个核苷酸的波形数据的峰值;数据集成部分,用于对多组波形数据进行集成处理。

著录项

  • 公开/公告号WO2014061146A1

    专利类型

  • 公开/公告日2014-04-24

    原文格式PDF

  • 申请/专利权人 HITACHI LTD.;

    申请/专利号WO2012JP77029

  • 发明设计人 YOKOI TAKAHIDE;ANAZAWA TAKASHI;

    申请日2012-10-19

  • 分类号G01N27/447;C12M1;C12N15/09;C12Q1/68;

  • 国家 WO

  • 入库时间 2022-08-21 15:50:15

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