PURIFICATION METHOD OF PROTEIN GROUP OF 30 KD IN SILKWORM PUPA

摘要

Disclosed is a purification method of a protein group of 30 kD in silkworm pupa, belonging to the technical field of purification of protein. The method comprises the purification steps: carrying out cell disruption on silkworm pupa, taking supernatant to conduct ammonia sulfate fractional precipitation after centrifugation of cell disruption liquid, suspending the sediment again and utilizing an ultrafiltration membrane to filter substances having a molecular weight of 80kD to 100kD, utilizing the ultrafiltration membrane to filter flow liquid having a molecular weight of 30kD to 50kD, collecting retaining liquid, and finally obtaining the protein group of 30 kD. The purification method of the present invention reduces damage and loss on interest protein, and the simplest steps can be utilized to obtain large amount of purified interest protein. The purification method is simple in operation and low in cost.