首页> 外国专利> COMPOSITION COMPRISING RECOMBINANT FUSION PROTEIN OF PATHOGENIC ANTIGEN PROTEIN AND FLAGELLIN OF VIBRIO VULNIFICUS FOR PREVENTING, ALLEVIATING, OR TREATING AGING

COMPOSITION COMPRISING RECOMBINANT FUSION PROTEIN OF PATHOGENIC ANTIGEN PROTEIN AND FLAGELLIN OF VIBRIO VULNIFICUS FOR PREVENTING, ALLEVIATING, OR TREATING AGING

机译：包含重组弧菌融合蛋白和 Vibrio Vulnificus 的弗拉格林的组合融合蛋白，用于预防，缓解或治疗衰老

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The present invention relates to a composition for preventing, alleviating, or treating aging, wherein the composition comprises a recombinant protein, as an active component, which fuses a flagellin, a component of a flagellum of Vibrio vulnificus, and a protein antigen of a pathogen. According to the present invention, using the recombinant protein of the present invention is advantageous for reducing the effects of external and internal function deterioration brought about by aging, and for strengthening immunity. Also, the composition of the present invention can easily induce immunity through mucosal administration. 展开▼

机译：本发明涉及一种用于预防，减轻或治疗衰老的组合物，其中该组合物包含重组蛋白作为活性成分，其融合鞭毛蛋白，即鞭毛弧菌的一种组分，和病原体的蛋白质抗原。根据本发明，使用本发明的重组蛋白有利于减少由衰老引起的外部和内部功能恶化的影响，以及增强免疫力。另外，本发明的组合物可以通过粘膜给药容易地诱导免疫。展开▼

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公开/公告号WO2014084456A1

专利类型

公开/公告日2014-06-05

原文格式PDF

申请/专利权人 INDUSTRY FOUNDATION OF CHONNAM NATIONAL UNIVERSITY;
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申请/专利号WO2013KR02547

发明设计人 CHO KYUNG A;LIM JAE SUNG;RHEE JOON HAENG;
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申请日2013-03-27

分类号A61K38/17;A61K38/16;A61P19;A61P1;

国家 WO

入库时间 2022-08-21 15:49:24

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1. 新型融合蛋白和用于预防或治疗癌症的包含该融合蛋白的药物组合物 [P] . 中国专利： CN112041333A . 2020-12-04

2. 用于预防和治疗癌症或血管生成相关疾病的药物组合物，其含有作为有效成分的融合蛋白，该融合蛋白融合了肿瘤穿透肽和抗血管生成剂 [P] . 中国专利： CN108348575A . 2018-07-31

3. COMPOSITION COMPRISING RECOMBINANT FUSION PROTEIN OF PATHOGENIC ANTIGEN PROTEIN AND FLAGELLIN OF VIBRIO VULNIFICUS FOR PREVENTING, ALLEVIATING, OR TREATING AGING [P] . 美国专利： US2020031879A1 . 2020-01-30

机译：包含病原菌蛋白和弗拉格菌弧菌素重组融合蛋白的组合物，用于预防，缓解或治疗老化

4. COMPOSITION COMPRISING RECOMBINANT FUSION PROTEIN OF PATHOGENIC ANTIGEN PROTEIN AND FLAGELLIN OF VIBRIO VULNIFICUS FOR PREVENTING, ALLEVIATING, OR TREATING AGING [P] . 美国专利： US2018118792A1 . 2018-05-03

机译：包含病原菌蛋白和弗拉格菌弧菌素重组融合蛋白的组合物，用于预防，缓解或治疗老化

5. COMPOSITION COMPRISING RECOMBINANT FUSION PROTEIN OF PATHOGENIC ANTIGEN PROTEIN AND FLAGELLIN OF VIBRIO VULNIFICUS FOR PREVENTING, ALLEVIATING, OR TREATING AGING [P] . 美国专利： US2016083437A1 . 2016-03-24

机译：包含病原菌蛋白和弗拉格菌弧菌素重组融合蛋白的组合物，用于预防，缓解或治疗老化

1. An efficient fusion protein system for expression ofBacillus anthracis protective antigen as immunogenic and diagnostic antigen [J] . Vahid Bagheri, Hossein Motamedi, Masoud Reza Seifiabad Shapouri 亚太热带医药杂志（英文版） . 2010,第010期

2. Enhancement of antigen-specific humoral immune responses and protein solubility through conjugation of bacterial flagellin, Vibrio vulnificus FlaB, to the N-terminus of porcine epidemic diarrhea virus surface protein antigen S0 [J] . Oh Seo-ho, Cho Young-Saeng Kim, Lee Ho-Bin, Journal of Veterinary Science . 2019,第6期

3. Enhancement of antigen-specific humoral immune responses and protein solubility through conjugation of bacterial flagellin, Vibrio vulnificus FlaB, to the N-terminus of porcine epidemic diarrhea virus surface protein antigen S0 [J] . Seo-ho Oh, Young-Saeng Kim Cho, Ho-Bin Lee, Journal of Veterinary Science . 2019,第6期

4. Phase 1 Safety and Immunogenicity Study of a Quadrivalent Seasonal Flu Vaccine Comprising Recombinant Hemagglutinin-Flagellin Fusion Proteins [J] . C. Jo White, Casey Johnson, Cynthia Strout, Open Forum Infectious Diseases . 2016,第1期

5. The antigenicity of the flagellin, outer membrane proteins and lipopolysaccharide of Vibrio harveyi [C] . Ying-xue Qin, Qing-pi Yan 2010 International Conference on Bioinformatics and Biomedical Technology (ICBBT 2010) . 2010

6. Isoniazid Activation by Mycobacterium tuberculosis Catalase-Peroxidase R104A/H108A Double Mutant - Kinetics and Emission Spectra of Vibrio harveyi Luciferase-Discosoma sp. Fluorescent Protein Fusion Proteins [D] . Ke, Dachuan 2011

7. Enhancement of antigen-specific humoral immune responses and protein solubility through conjugation of bacterial flagellin Vibrio vulnificus FlaB to the N-terminus of porcine epidemic diarrhea virus surface protein antigen S0 [O] . Seo-ho Oh, Young-Saeng Kim Cho, Ho-Bin Lee, 2019

8. Enhancement of antigen-specific humoral immune responses and protein solubility through conjugation of bacterial flagellin, Vibrio vulnificus FlaB, to the N-terminus of porcine epidemic diarrhea virus surface protein antigen S0 [O] . Seo-ho Oh, Young-Saeng Kim Cho, Ho-Bin Lee, 2019

1. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004）（P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期

2. Oral administration of a non-absorbable plant cellexpressed recombinant anti-TNF fusion protein induces immunomodulatory effects and alleviates nonalcoholic steatohepatitis [J] . Yaron Ilan ,Ami Ben Yaacov ,Yehudit Shabbat . 世界胃肠病学杂志：英文版 . 2016,第39期

3. Construction of expression systems for flaA and flaB genes of Helicobacter pylori and determination of immunoreactivity and antigenicity of recombinant proteins [J] . JieYan ,Shao-HuiLiang ,Ya-FeiMao . 世界胃肠病学杂志：英文版 . 2003,第10期

4. Hsp70-1ike protein 1 fusion protein enhances induction of carcinoembryonic antigen-specific CD^8＋ CTL response by dendritic cell vaccine [J] . Wu Y Wan T Zhou X Wang B Yang F Li N Chen G Dai S Liu S Zhang M Cao X . 第二军医大学学报 . 2006,第1期

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6. 表达Survivin-TERT融合蛋白的重组痘苗病毒的构建及其恶性肿瘤治疗研究 [A] . 王明龙 . 2019