THREE‑DIMENSIONAL CULTURE PERFUSION CHAMBER FOR TISSUE ENGINEERING

摘要

The present technology describes a perfusion chamber for tissue culture, the chamber being made of a material that can include different types of polymer that have good thermal and electrical properties and moderate strength, and that are easy to machine and capable of being autoclaved, which is an essential factor when the intention is to work with biological material. In terms of the physical aspect thereof, the chamber may have different formats, provided the conditions for culture of the tissue of interest are complied with. The perfusion chamber developed makes it possible to achieve three‑dimensional culture of cells seeded in a matrix that, when correctly adapted to the perfusion chamber, promotes the passage of the flow through this scaffold, guaranteeing that the medium is able to reach the majority of the cells, if not all the cells, thereby promoting cell viability and proliferation.