METHOD FOR KILLING AND TRACING BACTERIA BY COATING SAME WITH SELF-ASSEMBLED GOLD NANOSHELL LAYER AND PRODUCING PHOTOTHERMAL DECOMPOSITION AND COLD LIGHT BY MEANS OF LASER

摘要

Disclosed is a method for killing and tracing bacteria by coating same with a self-assembled gold nanoshell layer and producing photothermal decomposition and cold light by means of laser, which is capable of killing gram-positive bacteria, gram-negative bacteria and drug resistant bacteria. The present invention may produce a gold nanoshell layer on the surface of a bacterium by self-assembling without adding a reducing agent. Since gold has very good photothermal conversion efficiency, the bacterial gold nanomaterial can be irradiated with linear and continuous lasers and nonlinear femtosecond lasers to produce heat and effectively kill the bacterium, which is also known as the photothermal decomposition effect; when irradiating with the nonlinear femtosecond laser, the bacterial nanomaterial may not only produce thermal energy, but also has an optical characteristic of producing cold light, with more bacteria being killed the stronger the produced cold light, and sustainable shining not producing photobleaching; and the cold light can be used as a tracer agent to trace and confirm the survival, trend and located position of the bacteria.