METHOD FOR DETERMINING INTRINSIC BINDING PARAMETERS OF AN ANALYTE TO A LIGAND, A METHOD FOR SELECTING AN ANALYTE FROM A GROUP OF ANALYTS, THE SELECTED LIGAND OR ANALYTE, AND SENSOR

摘要

The present invention relates to a method for determining intrinsic binding parameters, such as K_D, k_d and k_a, of an analyte to a ligand, such as a drug and a protein, a drug and a receptor, and an antibody and antigen, wherein the maximal binding response R_max or R_L and at least one binding parameter is determined at at least two different ligand surface densities present on a sensor support, and extrapolating the value of the binding parameter to ligand density = 0, characterized by Rmax=0 or R₁=0, to a method of selecting an analyte and/or ligand, and to the selected ligand, analyte and a sensor.