MULTIPLEX ASSAY FOR TARGET SUBSTANCE WITH ENHANCED SIGNAL AMPLIFICATION USING GOLD NANOPARTICLES AND SYSTEM FOR THE SAME

摘要

Disclosed are multiplexing analysis method for target substance with enhanced signal amplification using gold nanoparticles and a system for the same. The analysis system for the target substance comprises: a supporter; a captor which is fixated to the supporter, and which selectively combines with the target substance; gold nanoparticle complex, fluorescent RNA finder, and ribonucleic acid lyase H. In the complex, the detector, as a different substance from the captor which selectively combines with the target substance, combines with the surface of the gold nanoparticle. In other region of the gold nanoparticle, a plurality of single strand of same deoxyribonucleotides is connected. The fluorescent RNA finder is inherent in a detector-gold nanoparticle complex and includes single strand of ribonucleotides to which fluorophore and quencher for the fluorophore are connected at the end. In the ribonucleotide, hybridization of ribonucleotide including complementary sequence of the deoxyribonucleotide with the above ribonucleotide is occured and fluorescent signals are generated and amplified by hydrolyzing the hybridized output using ribonucleic acid lyase H. [Reference numerals] (AA) First fluorescence;(BB) Second fluorescence;(CC) First fluorescent RNA probe;(DD) Second fluorescent RNA probe;(EE) First deoxyribonucleotides;(FF) Second deoxyribonucleotides;(GG) AFP detector-gold nanoparticle complex;(HH) FABP detector-gold nanoparticle complex