ORGANIC ACID PRODUCTION SYSTEM USING CARBONIC ANHYDRASE AND PHOSPHOENOLPYRUVATE CARBOXYLASE

摘要

The present invention relates to an organic acid production system using carbonic anhydrase and phosphoenolpyruvate carboxylase. Provided are a method for producing oxaloacetic acid comprising: a bicarbonate production step for making CO2 react with H2O under the presence of the carbonic anhydrase; and an oxaloacetate production step for making the bicarbonate react with phosphoenolpyruvate?‹ under the presence of the phosphoenolpyruvate carboxylase, and a transformant, in which an expression vector comprising carbonic anhydrase (CA) and phosphoenolpyruvate carboxylase (PEPCase) is transformed to the host cell, for producing the oxaloacetic acid. The organic acid production system according to the present invention has advantages of mass-producing the organic acid with higher yield, continuously producing the organic acid, and being possibly combined with carbon dioxide reduction technology by using the carbonic anhydrase and phosphoenolpyruvate carboxylse simultaneously.;COPYRIGHT KIPO 2014;[Reference numerals] (AA) Before reaction;(BB) After reaction