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DIAGNOSTIC TEST FOR BACTERIAL PATHOGENS USING INTERNAL CONTROL BACTERIAL STRAIN

机译:内控细菌菌株对细菌性致病菌的诊断试验

摘要

The invention relates to a method for detecting the presence or absence of a bacterial pathogen in a biological sample obtained from a human or animal subject, using an internal control. In particular, the invention relates to a method for detecting the presence or absence of Streptococcus equi in an equine sample using a control bacterial strain as internal control for DNA extraction and PCR. The invention also relates to host cells (such as bacterial cells) and nucleic acids for use as internal standard in said method in addition to diagnostic kits comprising said host cells and nucleic acids.
机译:本发明涉及一种使用内部对照检测从人或动物受试者获得的生物样品中细菌病原体存在或不存在的方法。特别地,本发明涉及一种使用对照细菌菌株作为DNA提取和PCR的内部对照来检测马样品中马链球菌的存在或不存在的方法。除了包含所述宿主细胞和核酸的诊断试剂盒以外,本发明还涉及在所述方法中用作内标的宿主细胞(例如细菌细胞)和核酸。

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