OLIGONUCLEOTIDE FOR DETECTING MUTATIONS IN GENES RELATED TO KOREAN NONSYNDROMIC HEARING LOSS, DNA CHIP, DIAGNOSTIC KIT AND DETECTION METHOD

摘要

The present invention relates to an oligonucleotide having a sequence number of 1-56 for detecting mutations in genes related to Korean nonsyndromic hearing loss or a complementary oligonucleotide, a DNA chip, a diagnostic kit, and a detection method using the oligonucleotide. By utilizing the present invention: detection of mutations in a plurality of specimens can be performed at the same time under the same condition by one experiment; detection can be completed rapidly, accurately, and simply; the DNA chip and the diagnostic kit, which include target probes capable of hybridizing specifically for each natural form and mutant form in order to detect mutations in genes related to the Korean nonsyndromic hearing loss, can be provided; and target products from mutations in genes (GJB2, SLC26A4, MT-RNA1) related to the Korean nonsyndromic hearing loss which are distributed over various locations can be amplified by minimum samples, reagents, and experimental manipulations. Also, diagnosis of mutations in specific part of the genes related to the Korean nonsyndromic hearing loss and quality inspection for DNA chips from after probe fixation of the DNA chips to after the hybridization can be facilitated by one experiment with a QC probe, and mutations distributed over various locations can be detected by one experiment.;COPYRIGHT KIPO 2014;[Reference numerals] (AA) Empty space