首页> 外国专利> IDENTIFYING METHOD OF DIATOMS IN SOUTHERN SEA OF KOREA, POLYNUCLEOTIDE PROBE, DNA CHIP AND KIT FOR IDENTIFYING THE SAME

IDENTIFYING METHOD OF DIATOMS IN SOUTHERN SEA OF KOREA, POLYNUCLEOTIDE PROBE, DNA CHIP AND KIT FOR IDENTIFYING THE SAME

机译:韩国南部海藻的鉴定方法,多核苷酸探针,DNA芯片和成套工具的鉴定方法

摘要

The present invention relates to an identification method of species of diatoms inhabiting the coast of Korea, and a polynucleotide probe, a DNA chip and a kit for identification for species of diatoms for the method. The identification method comprises the following steps: carrying out polymerase chain reaction (PCR) of the DNA extracted from diatoms to obtain a PCR product; binding the PCR product to each probe comprising a base sequence identical or complementary to one or more DNA sequences of sequence numbers 3-51; and identifying the species of the diatoms according to whether the PCR product binds or not. The present invention can analyze the genotype of diatoms inhabiting the coast of Korea and can simply, rapidly and accurately identify the species thereof on the basis of single nucleotide polymorphism (SNPs) of various species of diatoms.
机译:本发明涉及居住在韩国海岸的硅藻种类的鉴定方法,以及用于该方法的多核苷酸探针,DNA芯片和用于鉴定硅藻种类的试剂盒。鉴定方法包括以下步骤:对从硅藻提取的DNA进行聚合酶链反应(PCR),得到PCR产物。将PCR产物结合到每个探针上,所述探针包含与一个或多个序列号3-51的DNA序列相同或互补的碱基序列;根据PCR产物是否结合,鉴定硅藻的种类。本发明可以分析居住在韩国海岸的硅藻的基因型,并且可以基于各种硅藻的单核苷酸多态性(SNP)简单,快速和准确地识别其种类。

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