METHOD FOR MANUFACTURING TRANSGENIC PLANT PRODUCING TARGET PROTEIN IN LARGE QUANTITIES IN CHLOROPLAST AND PLANT PRODUCED THEREBY

摘要

The present invention provides: a recombinant vector for targeting a target protein comprising any one base sequence among the base sequences coding four kinds of transit peptides derived from PsbP protein, a kind of oxygen evolving protein (OEC) of tobacco, to a chloroplast of a plant; plant cells transformed with the recombinant vector; a method for producing the target protein in large quantities in the chloroplast of the plant by transferring and accumulating the target protein to the chloroplast of the plant, the method comprises a step for transforming the plant cells with the recombinant vector; a method for manufacturing a transgenic plant producing the target protein in large quantities in the chloroplast, the method comprises a step for transforming the plant cells with the recombinant vector; the transgenic plant producing the target protein produced by the same method in large quantities in the chloroplast; a method for producing the target protein in large quantities in the chloroplast by transferring and accumulating the target protein from a plant tissue to the chloroplast, the method comprises a step for transiently expressing the target protein by introducing the recombinant vector into the plant tissue; and a composition for targeting the chloroplast of the plant producing the target protein comprising any one base sequence among the base sequences coding four kinds of the transit peptides.;COPYRIGHT KIPO 2014