The present invention provides a method of cultivating Lingshi mushrooms using a multi-layer structure for cultivating Lingshi mushrooms by seeding and culturing Lingshi mushroom spawn. The method includes: a culturing preparation step of seeding Lingshi mushroom spawn into mediums such as a solid wood; a culturing step of culturing the spawn while keeping the mediums into which the Lingshi mushroom spawn is seeded in a room with a temperature of 20C to 28C; a bedding step of, when the culturing is completed by 85% to 95% in the culturing step, placing the mediums to a multi-layer structure including at least two layers installed in a cultivation facility; a pining management step of maintaining the temperature at 23C to 33C and supplying moisture at least once a day after the pining step before pilei are formed through initial pinning and complete pinning; and a final growing step of maintaining the temperature at 26C to 33C and supplying moisture at least once a day after the pinning management step until the Lingshi mushrooms with the pilei grow enough to harvest. Using the provided method of cultivating Lingshi mushrooms, it is possible to harvest Lingshi mushrooms twice or more than the existing method.
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