Process for Purification of Recombinant Human Chymotrypsinogen Ｂ２ in Transgenic Rice Cell Suspension Culture

摘要

The present invention provides transgenic rice cells with recombinant human trypsinogen expression in cultured liver suspension (Chymotrypsinogen) B2 As a method for purifying, (a) a refrigerated cell culture in order to remove most of the protein exists a- amylase (amylase) in rice cell suspension cultures in 4 for 12 hours and then dropped to pH 3 by a strong acid After centrifugation step; (B) a concentration step by ultrafiltration to exclude the molecular weight of 10,000Da size than the pH adjusted culture medium in the centrifuge step; And (c), and the human-derived chymotrypsin cyanogen B2 adsorbed on strong acid cation exchanger (strongly acidic cation exchanger) is Sulphopropyl-Sepharose from the concentrate obtained in the concentration step includes the step of elution with a single peak. Method for purifying recombinant human trypsinogen B2 chemokines expressed in transgenic rice cell suspension cultures according to the invention is just as economical purification process consisted of three steps therefore simplified, and is easily purified from the culture medium mass, average 90% has excellent effects that can be reproducibly purified purity. ;