cultivating method for mass production of tomato callus, and preparing method of extracts of tomato callus

摘要

The present invention relates to an anti-inflammatory or anti-allergic composition containing a tomato callus extract, a massive culture and production method of tomato callus, and a method for preparing the tomato callus extract. Anti-inflammation and anti-allergic activities are maximized by culturing pericarp of tomatoes in MS medium, inducing tomato callus, proliferating the tomato callus, and culturing and producing a large amount of tomato callus using an air lift bioreactor. The tomato callus extract with anti-inflammation and anti-allergic activities is prepared. The tomato callus extract has anti-inflammation and anti-allergic effects using human-derived keratinocyte and mouse-derived Raw 264.7 macrophage, such as cytotoxicity reduction, suppression of IL-1alpha and IL-6 expression, total No generation, and beta-hexosaminidase A generation and is used in a composition for preventing and alleviating inflammation and allergy, thereby being used as external use cosmetic materials with various forms.