APPARATUS AND METHOD FOR SIMULTANEOUSLY QUANTIFYING THE BINDING KINETICS AND REFRACTIVE INDEX OF MOLECULAR INTERACTIONS

摘要

The present invention relates to a device and a method for simultaneously measuring the molecular binding characteristics of low molecular bio substances and the refractive index of a buffer solution using an ellipsometry under an immersed microchannel environment and, more specifically, to a device and a method for simultaneously measuring the refractive index of a buffer solution and the dynamic binding characteristics of bio substances at high sensitiveness by introducing polarized incident beams to an adsorption layer formed of the bio substances on substrate substances for semiconductors using a prism structure and a microchannel under p-wave non-reflective conditions. The device comprises: a microchannel structure; a sample injection part for forming the adsorption layer of a sample on a substrate by injecting the buffer solution containing the sample of the bio substances to the microchannel; a polarized beam generation part for radiating the incident beams polarized through the incident surface of a prism to the adsorption layer at an incident angle satisfying the p-wave non-reflective conditions; and a polarized beam detection part for detecting the polarized beam variation of reflected beams after the reflected beams of the adsorption layer are introduced through the reflection surface of the prism. The microchannel structure comprises: the substrate composed of a support, and semiconductors or dielectric substances formed on the support; a cover part formed as the prism structure and installed in the support; and the microchannel formed on one among the upper part of the support and the bottom of the cover part.