The recombinant protein containing antigenic determinant of Helicobacter pylori urease to which IgA antibodies are reactive and its methods

摘要

The present invention relates to a method for diagnosing Helicobacter pylori infection in infants and young children, which comprises using a Helicobacter pylori urease antigen-crystal-based combination protein (hereinafter referred to as " Helicobacter pylori urease antigen- And a manufacturing method thereof.;By mapping the Helicobacter pylori antigenic determinants by two-dimensional electrophoresis and immunoblot analysis, the urease strongly reacts with the IgA antibody, thereby enhancing the specificity in the serological diagnosis and facilitating the purification of the antigen component . In the diagnosis of Helicobacter pylori infection in infants and young children, it is possible to distinguish serologically from antibodies transmitted from the mother to improve the healthcare of infants and young children.;Helicobacter pylori, recombinant protein, urease, IgA antiserum, serological diagnosis, infant and child diagnosis