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Fluorescence Polarization Assays for Determining Clostridial Toxin Activity
Fluorescence Polarization Assays for Determining Clostridial Toxin Activity
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机译:荧光偏振测定法测定梭菌毒素活性
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摘要
The present invention is a sample under conditions suitable for the (a) Clostridial toxin protease activity, fluoro pores; Group bulking (bulking group); And fluoro and processing cleavage site Clostridial toxin substrate comprises a Clostridial toxin recognition sequence comprising, between the fore and the bulking group; (B) exciting the fore as a fluoroalkyl plane polarized light (exciting) and; (C) by the fluorescence polarization of the treated substrate compared to the control substrate of crystal, the change in fluorescence polarization as compared to fluorescence polarization of the treated substrate of the control substrate indicates the presence or activity of a Clostridial toxin, Claus tree It provides a method of determining the presence or activity of toxin-based Stadium.
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