Variation Enzyme of Thermostable Bacillus circulans Xylanase and Gene thereof

摘要

the invention thermal stability is increased mutated Bacillus circulans xylanase enzyme (the BCX) relates to the three kinds of enzymes and enzyme combinations thereof , and their genes . More particularly, the present invention is a species of Bacillus circulans 64th amino acid glycine to alanine substitution of the xylanase enzyme gene and one mutated enzyme , histidine and 156th amino acid substitution of a valine enzyme gene mutation , substitution of the amino acid valine to isoleucine 168th and the enzyme is a mutant gene or mutation consisting of a combination of the enzymes and genes. Thermal stability is expected to be available in industrially useful mutant enzymes was increased as the reaction .