METHOD OF ASSESSMENT INFORMATION EFFECT FACTOR IN PHYSICAL EXPERIMENT

摘要

A method of evaluating the effect of a physical factor information in an experiment, wherein the first group of animals, such as rats, are applied to the thymus, conduct exposure to electromagnetic millimeter waves (EHF) at a wavelength of 5.6 mm in the region of the thymus for 2 weeks with intermittent 1-2 days, single exposure wherein the physical factor is 1-2 minutes after completion of EHF preventive procedures performed surgical simulation of surgery by opening and suturing the peritoneum to sed th day performed threefold fractionated external gamma irradiation of animals in the course dose 2.5 Sv (total dose 7.5 Sv), and then injected intraperitoneally cyclophosphamide at a dose of 4 mg / 100 g of animal body weight, na14 day after injection cytostatic carried slaughter animals with the study of blood and immune organs, thus determine cellularity of the thymus (CT), functional activity of lymphocytes in the test with nitroblue tetrazolium (NBT) in units. ., And the contents of antibody-forming cells (AFC) on NKErne in cells / PE in the spleen apoptosis in anneksinovom test (AP), the contents of circulating immune complexes, serum (CEC) units, after which the index of the effect of the physical factor (IF) by the formula: and when its value is less than 48 ascertain the effect the presence of a physical factor.