RECOMBINANT STRAIN OF BACTERIA Escherichia coli - PRODUCER OF AMBER ACID (VERSIONS) AND METHOD OF OBTAINING AMBER ACID USING STRAIN

摘要

1. A recombinant strain of bacteria Escherichia coli SGM1.0 [pPYC] - producing succinic acid having inactivated genes ackA, pta, poxB, ldhA, adhE and piruvatkarboksilazy.2 activity. A recombinant strain of bacteria Escherichia coli according to claim 1, characterized in that the pyruvate carboxylase activity is provided by introducing into the cells a DNA molecule strain containing a gene encoding an enzyme having the activity classified as KF 6.4.1.1.3. A recombinant strain of bacteria Escherichia coli SGM1.1 [pPYC] - producing succinic acid having inactivated genes ackA, pta, poxB, ldhA, adhE, iclR and piruvatkarboksilazy.4 activity. A recombinant strain of bacteria Escherichia coli according to claim 3, characterized in that the pyruvate carboxylase activity is provided by introducing into the cells a DNA molecule strain containing a gene encoding an enzyme having the activity classified as KF 6.4.1.1.5. A recombinant strain of bacteria Escherichia coli SGM2.0 [pPYC] - producing succinic acid having inactivated genes ackA, pta, poxB, ldhA, adhE, enhanced expression of genes aseE, aceFv. IPDA, and activity piruvatkarboksilazy.6. A recombinant strain of bacteria Escherichia coli according to claim 5, characterized in that the pyruvate carboxylase activity is provided by introducing into the cells a DNA molecule strain containing a gene encoding an enzyme having the activity classified as KF 6.4.1.1.7. A recombinant strain of bacteria Escherichia coli according to claim 5, characterized in that the gene expression aseE, aceF and lpdA enhanced by replacing a nucleotide in the chromosome of the native promoter sequences controlling gene expression aceEF-lpdA operon with a stronger promotor.8. A recombinant strain of bacteria Escherichia coli SGM2.1 [pPYC]