Method for immobilizing biomolecules on the surface of nanoparticles of magnetically iron covered carbon shell

摘要

The method of immobilization of protein molecules on the surface of magnet iron nanoparticles coated carbon shell comprising powder reacting with dissolved water 4 karboksibenzoldiazony tosylate to form a covalent bond of organic functional groups with powder surface iron nanoparticles coated carbon shell further carried carbodiimide activation using systems: dicyclohexylcarbodiimide with N-hydroxysuccinimide in dimethyl sulfoxide or distilled water (DCC / NHS DMSO / HO) or 1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride with N-hydroxysuccinimide in water (EDC / NHS in HO) or phosphate buffered saline, carried covalent "crosslinking" of protein molecules with an activated COOH group in an aqueous or buffered medium.