PROCEDURE FOR BIPHASIC PREPARATION OF LIPOSOMES AND APPLICATION THEREOF IN MANUFACTURING DIAGNOSTIC REAGENTS

摘要

1. A method of two-phase liposome preparation, during which the non-polar organic phase containing a mixture of natural and synthetic phospholipids and not miscible polar aqueous (buffer) mixed phase and the obtained liposome emulsion having a particle size of 50-100 nm, characterized in that liposome emulsion prepared by the method (s) simple mechanical mixing, wherein the monolayer or bilayer structure of the liposomal membranes, comprising totally organic phase is created using a mixture of individual ipidnyh molecules dissolved in the nonpolar organic phase and representing a positive or zero curvature, such as phosphatidylcholine, phosphatidylserine, phosphatidylethanolamine, lysophosphatidic acid, lysophosphatidylcholine and smaller amounts of other phospholipids of natural origin at a concentration in the range of 0.5-1.5 g / ml , wherein the membrane surface of liposome emulsion individual structure thus obtained is suitable for attaching the active ingredient (s) .2 (s). A method according to claim 1, characterized in that the apolar organic phase, a mixture of chloroform and methanol in a ratio of 2: 1, where the pH = 2, moreover, in an aqueous polar phase provide a buffering effect organic compounds, preferably 0.025 M tricine value pH polar aqueous (buffer) phase set in the range between pH = 6 and pH = 7, preferably pH = 6.6 with 5M NaOH, and to this was added antibacterial NaNkonservant in a concentration of 0.05 M, where the nonpolar organic phase of smaller volume is added to the polar aqueous (buffer) phase bol larger volume, which is 5-50 times higher in the