STRAIN BACILLUS THE LENTUS- PRODUCER OF THE BACTERIOCIN-LIKE SUBSTANCE OF LENTOCIN-PS1 OF THE WIDE SPECTRUM OF ANTIMICROBIC ACTION AND METHOD OF OBTAINING BACTERIOCIN-LIKE SUBSTANCE OF LENTOCIN-PS1

摘要

FIELD: biotechnology.;SUBSTANCE: strain Bacillus lentus "GKPM-Obolensk" B-7150 - producer of bacteriocin-like substance of broad spectrum of antimicrobial action is proposed, having molecular weight according to data of SDS-PAGE of about 4 kDa. Also the method of obtaining bacteriocin-like substance is provided, comprising submerged cultivation of Bacillus lentus "GKPM-Obolensk" B-7150 at a temperature 29-30°C in carbohydrate-yeast medium based on inorganic source of amino nitrogen with a limit of oxygen and carbohydrate. Isolation of bacteriocin-like substance from acellular fluid is carried out using dichloromethane or silica gel type adsorbent, and from the surface of cells - using ethanol. Then the fractions are combined and dried.;EFFECT: strain has antagonistic activity against coliform and other gram-negative bacteria and some species of gram-positive microorganisms.;2 cl, 3 dwg, 5 tbl, 7 ex