Recombinant DNA encoding the mature platelet derived growth factor B HUMAN (PDGF-B), the recombinant plasmid pAS250213, provides the synthesis of PDGF-B CELLS Escherichia coli, and the strain E. COLI BL21 (DE3) / pAS250213 - PRODUCER recombinant PDGF-B

摘要

1. A recombinant DNA encoding a mature platelet-derived growth factor B of human (PDGF-B), the nucleotide sequence (SEQ ID №1) which is optimized for protein production in the cell of Escherichia coli.2. PAS250213 recombinant plasmid, containing the NdeI / XhoI DNA fragment with the nucleotide sequence SEQ ID №1, encoding a mature polypeptide PDGF-B, and the NdeI / XhoI fragment of plasmid rET23a, recombinant plasmid pAS250213 provides high level expression of PDGF-B in the cells of Escherichia coli.3. The recombinant strain E. coli BL21 (DE3) / pAS250213 - PDGF-B protein producer.;1。编码人成熟血小板衍生生长因子B(PDGF-B)的重组DNA，其核苷酸序列(SEQ ID_1)被优化用于大肠杆菌细胞中的蛋白质生产。PAS250213重组质粒含有NdeI/XhoI DNA片段，核苷酸序列SEQ ID_1，编码成熟多肽PDGF-B，质粒rET23a的NdeI/XhoI片段，重组质粒pAS250213在大肠杆菌细胞中提供高水平的PDFF-B表达。OLI。3。重组大肠杆菌BL21（DE3）/PAS250213-PDGF-B蛋白产生菌。