METHOD FOR ASSESSING FUNCTIONAL STATUS OF ENDOTHELIUM IN EXPERIMENTAL ANIMALS AFTER RECONSTRUCTIVE SURGERIES OF ABDOMINAL AORTA

摘要

FIELD: medicine.;SUBSTANCE: invention refers to assessing the functional status of the endothelium (FSE) of experimental animals after the reconstructive surgeries of an abdominal aorta. Substance of the method consists in the fact that the FSE is assessed by means of the initial analysis of biochemical blood assay, including nitrogen oxide (II), superoxide dismutase (SOD), malondialdehyde (MDA); that is followed by abdominal aorta alloplasty; the biochemical control is performed 6 month later; the animals are removed from the experiment; the histological examination of the aorta alloplasty region is assisted by the computed morphometry, and the correlation is calculated. If observing an increase of NO metabolites, the development of intimal thickening is considered to be more manifested that it does not excess the physiological values specific for the arterial wall recovery following the surgical management, while a decrease of NO metabolites shows the development of intimal hyperplasia; an increase of SOD activity and MDA level provides stating the active development of arterial intimal thickening.;EFFECT: more effective FSE assessment in the animals after the reconstructive surgeries of the abdominal aorta.;3 tbl, 5 dwg, 1 ex